赢发彩票代理

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新城疫病毒疫苗的构建与免疫机制综述
发布时间:2020-01-28

  摘要:新城疫病毒引起的新城疫是一种禽类急性接触性传染病,采用疫苗防治该病是当前研究的热点领域之一。HN/F蛋白是2种有效的疫苗候选分子,乳酸乳球菌、鼠伤寒沙门菌、单增李斯特菌、根癌农杆菌、鸡痘病毒、牛痘病毒、鸽痘病毒、禽红白血病病毒、禽副粘病毒3型、传染性法氏囊病病毒和传染性气管炎病毒等微生物经过改造后可成为理想的疫苗载体。本文综述了重组乳酸乳球菌(rLL-HN)、重组鼠伤寒沙门菌(rSt-F和rSt-HN)、重组单增李斯特菌(rLm-F)、重组根癌农杆菌(rAt-F)、重组鸡痘病毒(rFPV-HN、rFPV-F-gB和rFPV-F-HN-gB)、重组牛痘病毒(rVV-F)、重组鸽痘病毒(rPPV-F)、重组禽红白血病病毒(rAEV-HN)、重组禽副粘病毒3型(rAPMV-F)、重组传染性法氏囊病病毒(rIBDV-HN)和重组传染性气管炎病毒(rIBV-HN)等疫苗的构建及其免疫机制的研制现状。

  关键词:新城疫病毒; HN/F蛋白; 疫苗;

  作者简介: *李文桂(1967-),男,博士,研究员,从事病原微生物的分子生物学和分子免疫学研究,E-mail:cqliwengui@163.com;

  基金: 国家自然科学基金(30801052;30671083;30500423;30200239);

The status of research on microorganisms-based vaccine of Newcastle disease virus

  Abstract:赢发彩票代理Newcastle disease caused by Newcastle disease virus is one type of acute contagious infectious diseases in avians. Recently control of the disease by use of vaccines becomes a highlight of research. HN/F antigen is an effective candidate molecule of vaccine, and many microorganisms such as Lactococcus lactis, Salmonella typhimurium, Listeria monocytogenes, Agrobacteria tumefaciens, fowlpox virus, vaccinia virus, pigeonpox virus, avian erythroblastosis virus, avian paramyxovirus type 3, infectious bursal disease virus and infectious bronchitis virus may become ideal vectors after they are modified. This review outlines the status of the research on the constructions and immune mechanisms of rLL-HN, rSt-F, rSt-HN, rLm-F, rAt-F, rFPV-HN, rFPV-F-gB, rFPV-F-HN-gB, rVV-F, rPPV-F, rAEV-HN, rAPMV-F, rIBDV-HN and rIBV-HN.

  Keyword:Newcastle disease virus; HN/F protein; Vaccine;

赢发彩票代理   XINCHENGYIBINGDU(Newcastle disease virus,NDV)SHIQINFUZHANBINGDU1XING(Avian paramyxovirus type 1,APMV-1)DEZHONGYAOCHENGYUANZHIYI,KEZAIQINLEIYINQIXINCHENGYI,ZHUYAOBIAOXIANWEIYIHUXIDAOHEXIAOHUADAONIANMOCHUXUEWEIDIANXINGBINGBIANDEJIXINGJIECHUXINGCHUANRANBING,DUIQINLEIYANGZHIWEIHAIJIAODA。TONGCHANGCAIYONGYOUMIAOHUORUODUYIMIAOJINXINGMIANYIYUFANG,DANRENMENFAXIANYOUMIAODEMIANYIQIDUAN,CHENGBENGAO,SHIYONGBUBIAN,RUODUYIMIAOKEYINQITIZHONGJIANQING、CIFAXIJUNHUOBINGDUGANRANYIJIMIANYIYIZHI,RUODUYIMIAOHAINENGYINQIJIHUXIDAODEQINGWEIBINGBIAN,ZENGJIADUIQITABINGYUANDEYIGANXING,ZHEIJIUXUYAOJINYIBUYANZHIXINXINGYIMIAO。

赢发彩票代理   NDVDEJIYINZUBIANMA6ZHONGDANBAI:HEDANBAI(NP)、LINDANBAI(P)、LDANBAI、JIZHIDANBAI(M)、XUENINGSU-SHENJINGANSUANMEI(hemagglutinin-neuraminidase,HN)HERONGHEDANBAI(fusion protein,F)。QIZHONGHN、FHEMWEIYUBINGDUNANGMODEBIAOMIAN,HNDANBAIJUYOUXUENINGSUHESHENJINGANSUANMEIDEHUOXING,NENGGOUSHIBIESUZHUBIAOMIANDESHOUTI,RONGJIESHOUTI,TAHAIYUFDANBAIXIANGHUZUOYONG,CUJINNDVYUXIBAODERONGHE。FDANBAIJIEDAOBINGDUNANGMOYUSUZHUJIANGMODERONGHE,CUJINBINGDUDECHUANRUHEZAIXIBAOJIANDEGANRAN,TAMENKEYOUDAOSUZHUCHANSHENGZHONGHEKANGTI,JUYOULIANGHAODEMIANYIYUANXING[1,2,3],SHANGSHUJIEGUOTISHIXUENINGSU-SHENJINGANSUANMEIHERONGHEDANBAISHI2ZHONGYOUQIANTUDEDANBAIKANGYUAN。

  SUIRANWEISHENGWUCHANGWEIHAIRENLEI,DANJIEZHUFENZISHENGWUXUEJISHUDEGAIZAOKECHENGWEILIXIANGDEYIMIAOZAITI。TAMENKETONGGUOZIRANGANRANDETUJINGJIANGMUDEDNAPIANDUANZHUANDAOMIANYIYOUDAODEBUWEI,YOUQISHITEDINGDEMIANYIXIBAO,TONGGUOXIBAOBIAODABAKANGYUAN,TONGSHIKEYOUDAOJUBUNIANMOMIANYIHEQUANSHENMIANYIYINGDA。GUONEIWAIXUEZHEXIANHOUBAODAOLEZHONGZURUSUANRUQIUJUN(rLL-HN)、ZHONGZUSHUSHANGHANSHAMENJUN(rSt-FHErSt-HN)、ZHONGZUDANZENGLISITEJUN(rLm-F)、ZHONGZUGENAINONGGANJUN(rAt-F)、ZHONGZUJIDOUBINGDU(rFPV-HN、rFPV-F-gBHErFPV-F-HN-gB)、ZHONGZUNIUDOUBINGDU(rVV-F)、ZHONGZUGEDOUBINGDU(rPPV-F)、ZHONGZUQINHONGBAIXUEBINGBINGDU(rAEV-HN)、ZHONGZUQINFUZHANBINGDU3XING(rAPMV-F)、ZHONGZUCHUANRANXINGFASHINANGBINGBINGDU(rIBDV-HN)HEZHONGZUCHUANRANXINGQIGUANYANBINGDU(rIBV-HN)DENGDUOZHONGYIMIAO,BENWENJIANGZONGSHUTAMENDEGOUJIANJIQIMIANYIJIZHIDENGFANGMIANDEYANJIUJINZHAN。

  1、细菌介导的重组新城疫病毒

  1.1 重组乳酸乳球菌

  RUSUANRUQIUJUN(Lactococcus lactis,L. lactis)SHIYIZHONGLIXIANGDEYIMIAOZAITI,KECHENGGONGBIAODALUNZHUANGBINGDUDEVP4DANBAI[4].HUJINGTAODENG[5,6,7]YINDVJIYINZUDEZONGRNAWEIMOBANJINXINGRT-PCR,KUOZENGHNJIYIN,CHARUpMD18-TDEpMD18-HN,YUpSIP09ZHONGZUDEpSIP-HN,JIANGQIDIANCHUANKONGZHUANHUAZHIWURUGANJUN(Lactobacillus plantarum),200 μg/mLDEHONGMEISUSHAIXUANZHONGZURUSUANRUQIUJUN,YICONGrLL-HNTIQUDEZONGRNAWEIMOBAN,CAIYONGRT-PCRFANGFAKEKELONGCHU1 716 bpDEHVJIYINPIANDUAN,Western blotSHIYANFAXIANNDVGANRANXUEQINGKEJIEHErLL-HNBIAODADE66 kDaDEHNKANGYUAN,LIUSHIXIBAOYI(FACS)JIANCEFAXIANrLL-HNDEBIAOMIANCUNZAIHNDANBAI。JIANG1×109 CFUZHONGZUYIMIAOGUANWEIMIANYIBALB/cSHU,MEIZHOULIANXU3 d,JIANGE4 d,LIANXU2ZHOU。CISHIPIXIAZHUSHE5×105JIECHANGAIXIBAOZHUCT26-WT,5 dHOUKECHUJIZHONGKUAI。QU1ZHISHU,FENLIZHONGLIU,QIECHENG2 mm3XIAOKUAI,FUBUSHOUSHUJIANLIJIECHANGAIDEYUANWEIYIZHILIUMOXING,JIANMO1ZHOUHOUYONGYIMIAOMEIZHOUGUANWEI1CI,LIANXU3ZHOU,ZAIMIANYIZHILIAOHOU30 dFAXIANXIAOSHUZHONGLIUDETIJIMINGXIANSUOXIAOHUOTUIHUA,CUNHUOSHIJIANXIANZHEYANZHANG。

  1.2 重组鼠伤寒沙门菌

  SHUSHANGHANSHAMENJUN(Salmonella typhimurium,S. typhimurium)DEYINGYANGQUEXIANXINGTUBIANZHUSL7207HUOSL1344JUNKEZUOWEIYIMIAOZAITISHIYONG。PANZHIMINGDENG[8,9,10,11]YINDVZONGRNAWEIMOBANKUOZENGFJIYIN,CHARUpGEM-TDEpGEM-F,YUpVAX1ZHONGZUDEpVAX1-F,DIANCHUANKONGZHUANHUASHUSHANGHANSHAMENJUNJIANDUZHUSL7207,SHAIXUANYANGXINGZHONGZUJUN,YICONGZHONGZUJUNCHOUTIDEZONGRNAWEIMOBANJINXINGRT-PCRKEKUOZENGCHU1.7 kbDEFJIYIN,MIANYIYINGGUANGFAXIANZHONGZUJUNDEBIAOMIANCUNZAIFDANBAIKANGYUAN。QICHUCAIYONG109 CFUZHONGZUJUNMIAOKOUFUGUANWEIBALB/cSHU,ZAICHUCIJIEZHONGHOU2ZHOUJINXINGJIAQIANG,YANJIUBIAOMINGMIANYIZUDEXUEQINGIgGHEXIAOCHANGYESIgAZAICHUCIJIEZHONGHOU2~4ZHOUSHANGSHENG,ZAICHUCIJIEZHONGHOU4ZHOUDADAOJIAOGAOCHENGDU。JIEZHEJIANG109HUO108 CFUYIMIAOKOUFUMIANYI1 dLINGDESHANGPINDAIYISHAHEDANJI,ZAISHOUCIMIANYIHOU2ZHOUHE4ZHOUJIAQIANG2CI,ZAIMOCIMIANYIHOU10 dJIROUZHUSHE104DE50%PEITAIZHISIJILIANG(50% embryo lethal doses,ELD50)XINCHENGYIBINGDUF48E9ZHUJINXINGGONGJI,ZAIGONGJIHOU14 dFAXIAN109 CFUMIANYIZU、108 CFUMIANYIZUHEDUIZHAOZUDECUNHUOLVFENBIEWEI72.3%(17/22)、52.1%(12/23)HE12.5%(2/16)。

  TIANFANGHUADENG[12]YIpMD18-HNWEIMOBANKUOZENGHNJIYIN,CHARUpYA3493DEpYA3493-HN,JIANGQIDIANCHUANKONGZHUANHUASL1344△crop△asdZHU,ERANJIGENGERSUAN(diaminopimelic acid,DAP)SHAIXUANZHONGZUSHUSHANGHANSHAMENJUN,YICONGrSt-HNTIQUDEZONGRNAWEIMOBAN,CAIYONGRT-PCRFANGFAKEKELONGCHU1.7 kbDEHNJIYINPIANDUAN。SHANGKEDENG[13]JIANGpYA3493-HNZHILIDIANCHUANKONGZHUANHUAJIWENSHAMENJUN(Salmonella pullorum)DEJIANDUZHUC17-13△crop△asd,DAPSHAIXUANZHONGZUSHUSHANGHANSHAMENJUN,Western blotSHIYANFAXIANGANRANXUEQINGKESHIBIErSt-HNBIAODADE63 kDaDEHNDANBAI。LIMENGDENG[14]YIpMD18-HNWEIMOBANKUOZENGHNJIYIN,CHARUpcDNA3.0DEpcD-HN,JIANGQIDIANCHUANKONGZHUANHUAJIWENSHAMENJUNJIANDUZHU,SHAIXUANYANGXINGZHONGZUJUN,MIANYIYINGGUANGFAXIANZHONGZUJUNDEBIAOMIANCUNZAIHNDANBAIFENZI。

  1.3 重组单增李斯特菌

赢发彩票代理   YANJIUBIAOMINGDANZENGLISITEJUN(Listeria monocytogenes)SHIYIZHONGYIMIAOZAITI,KECHENGGONGBIAODARENRUTOULIUBINGDU16XINGDEE7DANBAI[15].XUJINGJINGDENG[16]YINDV F48E9ZHUJIYINZUDNAWEIMOBANKUOZENGFJIYIN,CHARUpUC18-hly,DEpUC18-hly-F,YUpKSV7ZHONGZUDEpKSV7-hly-F,JIANGQIDIANCHUANKONGZHUANHUADANZENGLISITEJUN10403SZHU,SHAIXUANZHONGZUDANZENGLISITEJUN,YICONGrLm-FTIQUDEZONGRNAWEIMOBAN,CAIYONGRT-PCRFANGFAKEKELONGCHU1 662 bpDEFJIYINPIANDUAN。JiangDENG[17]YIXINCHENGYIBINGDUF48E9ZHUDEJIYINZUDNAWEIMOBANKUOZENGJIEDUANDEFJIYIN(Fa),CHARUpUC18△PLcBDEpUC18△PLcB-Fa,YUpKSV7ZHONGZUDEpPAD-Fa,JIANGQIDIANCHUANKONGZHUANHUADANZENGLISITEJUN10403SZHU,SHAIXUANYANGXINGJUNZHU,YICONGZHONGZUJUNCHOUTIDEZONGRNAWEIMOBANJINXINGRT-PCRKUOZENGCHU1 kbDEFaJIYIN,Western blotSHIYANFAXIANGANRANXUEQINGKESHIBIEZHONGZUJUNBIAODADE19.2 kDaDEFaKANGYUAN。JIEZHEJIANG1×109 CFUYIMIAOKOUFUHUO1×108 CFUYIMIAOFUQIANGZHUSHEMIANYI10 dLINGDEZIJI,ZAISHOUCIMIANYIHOU1ZHOUJINXINGJIAQIANG,YANJIUBIAOMINGMIANYIZUDEXUEQINGIgGZAISHOUCIMIANYIHOU3~6ZHOUSHANGSHENG,ZAISHOUCIMIANYIHOU6ZHOUSHENGDAOJIAOGAOCHENGDU,ZAISHOUCIMIANYIHOU7ZHOUYONG105 LD50DEXINCHENGYIBINGDUF48E9ZHUJINXINGJIROUZHUSHEGONGJI,ZAIGONGJIHOU2ZHOUJISHUCUNHUOLV,FAXIANKOUFUZU、FUQIANGZHUSHEZUHEDUIZHAOZUDECUNHUOLVFENBIEWEI56.5%(13/23)、30.4%(7/23)HE0(0/19)。

  1.4 重组根癌农杆菌

  FraleyDENG[18]JIEZHUGENAINONGGANJUN(Agrobacteria tumefaciens)JIANGXIJUNJIYINDAORUZHIWUXIBAO,ZHEIWEIYANZHIZHUANJIYINZHIWUYIMIAOTIGONGLEKEXINGXING。YANGZHENQUANDENG[19]YIpVAX1-FWEIMOBANKUOZENGFJIYIN,CHARUpGEM-TDEpGEM-F,YUpYU158ZHONGZUDEpYU158-F,JIANGQICAIYONGDONGRONGFADAORUEHA105ZHU,RANHOUGANRANSHUIDAO(Oryza sativa)DEZIYE,CHENGGONGZAIPEIZHUANJIYINSHUIDAO,ELISAJIANCEZHUANJIYINZHIZHU,QIZHONG2/6ZHUCUNZAIFDANBAI,YICONGZHUANJIYINSHUIDAOCHOUTIDEDNAWEIMOBAN,CAIYONGPCRFANGFAKEKELONGCHU1.7 kbDEFJIYINPIANDUAN,Western blotBIAOMINGGANRANXUEQINGKEJIEHEZHUANJIYINSHUIDAOBIAODADE59 kDaDEFDANBAI。JIANG400 μgDEZHUANJIYINYEPIANKANGYUANDANBAITIANJIAFUSHIZUOJI,CAIYONGPIXIAZHUSHEDEFANGFAJIEZHONGBALB/cSHU,ZAICHUCIJIEZHONGHOU10 dJINXINGJIAQIANG,ZAICHUCIJIEZHONGHOU25 dBIAOMINGXUEQINGIgGSHANGSHENG,DIDUKEDA1∶139.3.

新城疫病毒疫苗

配图 新城疫病毒疫苗

  2、病毒介导的NDV疫苗

  2.1 重组鸡痘病毒

赢发彩票代理   JIDOUBINGDU(Fowlpox virus,FPV)DESUZHUFANWEIJINXIANYUQINLEI,JIYINZUQUANZHANG300 kbZUOYOU,DAYUEHANYOU250GEKAIFAYUEDUKUANG(ORF),CUNZAIDALIANGDEFUZHIFEIBIXUQU,KEZUOWEIBINGDUZAITIBIAODAWAIYUANJIYIN,KECHARU25~30 kbDEWAIYUANDNA,JIBENBUGAIBIANFPVDESHENGWUXUETEXING,BIAODADEMUDEDANBAIKEBEITANGJIHUAHUOLINSUANHUAXIUSHI,KEYOUDAOKANGTICHANSHENGXIBAOHETIYEMIANYIYINGDA,SHIYIZHONGLIXIANGDEYIMIAOZAITI。OgawaDENG[20]JIANGHNJIYINCHARUpNZ136DEpNZ1037,JIANGQIZHUANHUAFPV,RANHOUZHUANRANJIPEICHENGXIANWEIXIBAO(chicken embryo fibroblasts,CEF)ZHUDF1,SHAIXUANZHONGZUJIDOUBINGDU,Western blotSHIYANBIAOMINGGANRANXUEQINGKEJIEHErFPV-HNBIAODADE66 kDaDEHNKANGYUAN。YONG1.5×105 PFUZHONGZUFPV-HNCHINEIPIXIAZHUSHE7 dLINGDELAIHANGZIJI,ZAIJIEZHONGHOU2ZHOUTISHIXUEQINGIgGSHENGGAO,DIDUWEI1∶15.3,CISHIYONG3.4×105 CFUDENDV SatoZHUJINXINGJIROUZHUSHEGONGJI,ZAIGONGJIHOU2ZHOUJISHUCUNHUOLV,FAXIANMIANYIZUDECUNHUOLVWEI80%(8/10),ERDUIZHAOZUWEI0(0/5)。

  BoursnellDENG[21]YIXINCHENGYIBINGDUBCZHUDEZONGRNAWEIMOBANKUOZENGHNJIYIN,CHARUpUC19DEpUC19-HN,YUpB3MEZHONGZUDEpB3ME-HN,JIANGQIDAORUFPVHOU,SUIHOUGANRANDF1XIBAOZHU,SHAIXUANZHONGZUJIDOUBINGDU,Western blotSHIYANBIAOMINGGANRANXUEQINGKEJIEHErFPV-HNBIAODADE66 kDaDEHNKANGYUAN。YONG106、104HE102 PFUDErFPV-HNFENBIEJINGMAIZHUSHE3ZHOULINGDEZIJI,ZAIMIANYIHOU3ZHOUJIROUZHUSHE105 ELD50XINCHENGYIBINGDUYOUDUZHUJINXINGGONGJI,ZAIGONGJIHOU16 dFAXIAN106 PFUMIANYIZU、104 PFUMIANYIZU、102 PFUMIANYIZUHEDUIZHAOZUDECUNHUOLVFENBIEWEI100%(10/10)、60%(6/10)、30%(3/10)HE0(0/10)。

赢发彩票代理   IritaniDENG[22]JIANG104 PFUZHONGZUBINGDUCHINEIPIXIAZHUSHE3 dLINGDEZIJI,TISHIMIANYIZUDEXUEQINGIgGZAICHUCIJIEZHONGHOU1~6ZHOUSHANGSHENG,ZAICHUCIJIEZHONGHOU4ZHOUSHANGSHENGDAOJIAOGAOCHENGDU,DIDUDADAO1∶1 068,ZAISHOUCIJIEZHONGHOU62 dJIANG105 ELD50XINCHENGYIBINGDUSatoZHUJINXINGJIROUZHUSHEGONGJI,ZAIGONGJIHOU14 dJISHUCUNHUOLV,FAXIANMIANYIZUDECUNHUOLVWEI100%(10/10),ERDUIZHAOZUWEI0(0/5)。EdbauerDENG[23]JIANG104DE50%ZUZHIPEIYANGGANRANJILIANG(50% tissue-culture infectious doses,TCID50)ZHONGZUBINGDUJIROUZHUSHEHUODIANYANMIANYIZIJI,ZAISHOUCIMIANYIHOU4ZHOUJIAQIANG1CI,ZAISHOUCIMIANYIHOU7ZHOUJIROUZHUSHE105 ELD50XINCHENGYIBINGDUTexasZHUJINXINGGONGJI,ZAIGONGJIHOU2ZHOUFAXIANJIROUZHUSHEZU、DIANYANZUHEDUIZHAOZUDECUNHUOLVFENBIEWEI100%(10/10)、30%(3/10)HE0(0/10)。NagyDENG[24]JIANG104 PFUZHONGZUBINGDUCHINEIPIXIAZHUSHE4ZHOULINGDELAIHANGZIJI,ZAIMIANYIHOU3ZHOUFAXIANXUEQINGIgGSHENGGAO,CISHIJIROUZHUSHE105 ELD50XINCHENGYIBINGDUTexasZHUJINXINGGONGJI,ZAIGONGJIHOU2ZHOUFAXIANMIANYIZUHEDUIZHAOZUDECUNHUOLVFENBIEWEI90%(9/10)HE0(0/10)。KaracaDENG[25]JIANG125、250、500HE1 000 PFUZHONGZUBINGDULUANNEIZHUSHE14 dLINGDEFUHUAJIDAN,ZAIMIANYIJIEZHONGHOU14 dZIJIFUCHU,CISHIYONG104 ELD50XINCHENGYIBINGDUNDV-GB-TXZHUJINXINGDIANYANDIBIGONGJI,ZAIGONGJIHOU14 dFAXIAN125 PFUMIANYIZU、250 PFUMIANYIZU、500 PFUMIANYIZUHE1 000 PFUMIANYIZUHEDUIZHAOZUDECUNHUOLVFENBIEWEI71%(10/14)、73%(8/11)、86%(6/7)、100%(11/11)HE0(0/11)。

  CAODIANJUNDENG[26]JIANGpHN-PEFL-29DAORUFPV,RANHOUGANRANDF1XIBAO,SHAIXUANZHONGZUJIDOUBINGDU,Western blotSHIYANBIAOMINGGANRANXUEQINGKEJIEHErFPV-HNBIAODADE74 kDaDETANGJIHUAHNDANBAIHE68 kDaFEITANGJIHUADANBAI。JIANG105 PFU、104 PFUYIMIAOJIROUZHUSHEZIJI,TISHIMIANYIZUDEXUEQINGIgGZAIJIEZHONGHOU6~16 dSHANGSHENG,ZAIJIEZHONGHOU14 dSHANGSHENGDAOJIAOGAOCHENGDU,ZAIJIEZHONGHOU16 dJIROUZHUSHE3.4×105 PFUXINCHENGYIBINGDUF48E9ZHUJINXINGGONGJI,ZAIGONGJIHOU14 dFAXIAN105 PFUMIANYIZU、104 PFUMIANYIZUHEDUIZHAOZUDECUNHUOLVFENBIEWEI80%(8/10)、60%(6/10)、HE0(0/10)。LIUWEIZHONGDENG[27]JIANGHNJIYINCHARUpFG1175-11DEpFG-HN,ZHUANHUAFPVDE282E4ZHUHOUZAIZHUANRANDF1XIBAO,SHAIXUANZHONGZUBINGDU,NorthernZAJIAOFAXIANHNJIYINCHARUZHONGZUBINGDUDEJIYINZUZHONG。WUYANTAODENG[28]JIANG104 TCID50ZHONGZUBINGDUJIROUZHUSHE1 dLINGDEZIJI,ZAIMIANYIHOU6ZHOUJIROUZHUSHE105 EID50XINCHENGYIBINGDUF48E8ZHUJINXINGGONGJI,ZAIGONGJIHOU2ZHOUFAXIANMIANYIZUHEDUIZHAOZUDECUNHUOLVFENBIEWEI96.7%(29/30)HE0(0/5)。DINGWEIDONGDENG[29]JIANG105 PFUZHONGZUBINGDUJINGBUPIXIAZHUSHE1 dLINGDEZIJI,ZAIMIANYIHOU3ZHOUJIROUZHUSHE106 ELD50DEXINCHENGYIBINGDUF48E8ZHUJINXINGGONGJI,ZAIGONGJIHOU10 dFAXIANMIANYIZUHEDUIZHAOZUDECUNHUOLVWEI100%(10/10)HE0(0/10)。

赢发彩票代理   GANJUNJIDENG[30,31,32]JIANG2×104 PFUZHONGZUBINGDUJINGBUPIXIAZHUSHE1 dLINGDEZIJI,FAXIANXUEQINGIgGZAIMIANYIHOU1~3ZHOUSHENGGAO,ZAI1ZHOUDAJIAOGAOSHUIPING,ZAIMIANYIHOU3ZHOUYONG106 ELD50DEXINCHENGYIBINGDUF48E8ZHUJINXINGDIANYANDIBIGONGJI,FAXIANMIANYIZUHEDUIZHAOZUDECUNHUOLVFENBIEWEI90%(18/20)HE0(0/20)。JIEZHEYONG103 PFUDErFPV-HNJIEZHONG14 dLINGDEZIJI,ZAICHUCIJIEZHONGHOU4ZHOUJINXINGJIAQIANG,TISHIMIANYIZUDEXUEQINGIgGZAICHUCIJIEZHONGHOU1~7ZHOUSHANGSHENG,ZAICHUCIJIEZHONGHOU6ZHOUSHANGSHENGDAOJIAOGAOCHENGDU,ZAIDI2CIJIEZHONGHOU3、7、12HUO18ZHOUYONG106 ELD50XINCHENGYIBINGDUF48E8ZHUJINXINGGONGJI,FAXIANGEGEMIANYIZUDECUNHUOLVJUNWEI100%(10/10),ERDUIZHAOZUWEI0(0/10)。SUIHOUJIANG103 PFUZHONGZUBINGDUMIANYI14 dDEZIJI,TISHIMIANYIZUDEXUEQINGIgGZAIJIEZHONGHOU1~3ZHOUSHANGSHENG,ZAIJIEZHONGHOU2ZHOUSHANGSHENGDAOJIAOGAOCHENGDU,ZAIJIEZHONGHOU3ZHOUYONG105 ELD50XINCHENGYIBINGDUF48E8ZHUJINXINGGONGJI,ZAIGONGJIHOU14 dFAXIANMIANYIZUHEDUIZHAOZUCUNHUOLVFENBIEWEI100%(10/10)HE0(0/10)。

赢发彩票代理   CHUANRANXINGHOUQIGUANYANBINGDU(Infectious laryngotracheitis virus,ILTV)KEYINQICHUANRANXINGHOUQIGUANYAN,TANGDANBAIB(glycoprotein B,gB)YUBINGDUXIFUHEJINRUXIBAOYOUGUAN,SHIYIZHONGBAOHUXINGKANGYUAN[33].ZHIHAIDONGDENG[34,35,36,37]YIXINCHENGYIBINGDUDEJIYINZUDNAWEIMOBANKUOZENGFJIYIN,CHARUpSY538DEpSY-F,YUpSY781ZHONGZUDEpSY-F-gB,JIANGQIZHUANHUAFPVHOUZAIZHUANRANDF1XIBAO,SHAIXUANZHONGZUJIDOUBINGDU,YICONGrFPV-F-gBTIQUDEDNAWEIMOBAN,CAIYONGPCRFANGFAKEKELONGCHU549 bpDEFJIYINPIANDUANHE394 bpDEgBJIYINPIANDUAN,Western blotSHIYANBIAOMINGGANRANXUEQINGKEJIEHErFPV-F-gBBIAODADE110 kDaDERONGHEDANBAI。JIANG5×103 PFUYIMIAOCHINEIPIXIAZHUSHE1 dLINGDEZIJI,TISHIMIANYIZUDEXUEQINGTEYIXINGKANGTIZAIJIEZHONGHOU10~25 dSHANGSHENG,ZAIJIEZHONGHOU20 dSHANGSHENGDAOJIAOGAOCHENGDU,FACSFAXIANWAIZHOUXUEDANHEXIBAO(PBMC)DECD4+HECD8+YAQUNDESHUMUWUMINGXIANBIANHUA;ZAIMIANYIHOU4ZHOUJIROUZHUSHE100 EID50XINCHENGYIBINGDUF48E8ZHUHUOCHUANRANXINGHOUQIGUANYANBINGDUWGZHUJINXINGGONGJI,FAXIANNDVGONGJIZU、ILTVGONGJIZUHEDUIZHAOZUDECUNHUOLVFENBIEWEI80%(8/10)、100%(10/10)HE0(0/10)。

  SUNHUILINGDENG[38,39]YIXINCHENGYIBINGDUF48E9ZHUDEZONGRNAWEIMOBANKUOZENGHNJIYIN,CHARUpSY538DEpSY-HN,YUpSY538LPFZHONGZUDEpSY-HN-F,YUpSY781ZHONGZUDEpSY-HN-F-gB,JIANGQIDAORUFPV017ZHU,RANHOUGANRANDF1XIBAO,SHAIXUANZHONGZUJIDOUBINGDU,Western blotSHIYANBIAOMINGGANRANXUEQINGKESHIBIErFPV-F-HN-gBBIAODADE63 kDaDEHN、53 kDaDEFYIJI58 kDaDEgBDANBAI。SUIHOUJIANG103 PFUYIMIAOCHINEIPIXIAZHUSHE4ZHOULINGDELAIHANGJI,FAXIANXUEQINGHN、FHEgBTEYIXINGIgGZAIMIANYIHOU1~4ZHOUSHENGGAO,ZAIMIANYIHOU3ZHOUDAJIAOGAOSHUIPING;ZAIMIANYIHOU4ZHOUJIROUZHUSHE103 EID50XINCHENGYIBINGDUF48E9ZHUHUOQIGUANNEIJIEZHONG500 EID50CHUANRANXINGHOUQIGUANYANBINGDUWGZHUJINXINGGONGJI,ZAIGONGJIHOU10 dJISHUCUNHUOLV,FAXIANNDVGONGJIZU、ILTVGONGJIZUHEDUIZHAOZUDECUNHUOLVFENBIEWEI50%(5/10)、70%(7/10)HE0(0/10)。

  2.2 重组牛痘病毒

赢发彩票代理   SutterDENG[40]BAODAONIUDOUBINGDU(Vaccinia virus,VV)DEMVAJIANDUZHUKEBIAODADUOZHONGBINGYUANTIDEMUDEDENG。MeulemansDENG[41]JIANG15DE50%JIGANRANJILIANG(50% chicken infective doses,CID50)ZHONGZUVV-FCHINEIPIXIAZHUSHE15ZHOULINGDEZIJI,ZAIMIANYIHOU12 dFAXIANXUEQINGIgGSHENGGAO,CISHIJIROUZHUSHE106.5DE50%JIZHISIJILIANG(50% chicken lethal doses,CLD50)XINCHENGYIBINGDUItalienZHUJINXINGGONGJI,ZAIGONGJIHOU14 dFAXIANMIANYIZUHEDUIZHAOZUDECUNHUOLVFENBIEWEI100%(9/9)HE14.3%(1/7)。

  2.3 重组鸽痘病毒

赢发彩票代理   GEDOUBINGDU(Pigeonpox virus,PPV)SHIYIZHONGQINDOUBINGDU,KEZUOWEIBIAODAWAIYUANKANGYUANDEYIMIAOZAITI。LetellierDENG[42]JIANGFJIYINCHARUpTK1DEpTK-F,JIANGQIZHUANHUAPPV△TKZHUHOUZAIZHUANRANDF1XIBAO,YONG25 μg/mLDE5-XIU-2′-TUOYANGNIAOGAN(BUdR)SHAIXUANZHONGZUBINGDU。JIANG104.33 TCID50YIMIAOLVPAOHUODIANYANDIBIJIEZHONG6ZHOULINGDEZIJI,ZAIMIANYIHOU20 dFAXIANXUEQINGIgGSHENGGAO,DIDUFENBIEWEI1∶4 011HE1∶483,CISHIYONG106 ELD50XINCHENGYIBINGDUItalianZHUDIANYANDIBIJINXINGGONGJI,ZAIGONGJIHOU4ZHOUFAXIANLVPAOJIEZHONGZU、DIANYANDIBIJIEZHONGZUHEDUIZHAOZUDECUNHUOLVFENBIEWEI94.4%(17/18)、50%(11/22)HE0(0/10)。

  2.4 重组禽红白血病病毒

  QINHONGBAIXUEBINGBINGDU(Avian erythroblastosis virus,AEV)SHIYIZHONGNIZHUANLUBINGDU,KEJIEZHUpNHn3ZAITIBIAODAMUDEJIYIN。CossetDENG[43]JIANGHNJIYINCHARUpNHn3DEpNHn3-HN,JIANGQIZHUANHUAAEVHOUZAIZHUANRANDF1XIBAO,SHAIXUANZHONGZUBINGDU,MIANYIYINGGUANGFAXIANZHONGZUBINGDUDEBIAOMIANCUNZAIHNDANBAIFENZI。JIANG107 PFUZHONGZUBINGDUJIAFUSHIZUOJIJIROUZHUSHE1ZHOULINGDEZIJI,ZAISHOUCIMIANYIHOU2ZHOUJIAQIANG1CI,ZAISHOUCIMIANYIHOU5ZHOUJIROUZHUSHE105.3 EID50XINCHENGYIBINGDUTexasZHUJINXINGGONGJI,ZAIGONGJIHOU2ZHOUFAXIANMIANYIZUHEDUIZHAOZUDECUNHUOLVFENBIEWEI100%(10/10)HE0(0/10)。

  2.5 重组禽副粘病毒3型

赢发彩票代理   QINFUZHANBINGDU3XING(Avian paramyxovirus type 3,APMV-3)SHIYIZHONGRNABINGDU,JINZAIBAOJIANGFUZHI,BUZHENGHERUSUZHUXIBAODEJIYINZUZHONG,JINBIANMA8ZHONGDANBAI,DUIMIANYIYINGDADEYINGXIANGJIAOXIAO,JINGANRANQINLEI,DUIRENTIWUHAI,KETONGGUODIANYANDIBIJIEZHONG,GANRANJINJUXIANYUHUXIDAO,KEZAIJIPEIHUOXIBAOZHUZHONGCHANSHENGGAODIDUDEBINGDU[44].KumarDENG[45]JIANGFJIYINCHARUpAPMV3f1DEpAPMV-F,JIANGQIDAORUAMPV3,RANHOUGANRANDF1XIBAO,SHAIXUANZHONGZUQINFUZHANBINGDU3XING,Western blotSHIYANBIAOMINGGANRANXUEQINGKEJIEHErAPMV-FBIAODADE55 kDaDEFDANBAI。JIANG106 EID50YIMIAODIANYANDIBIMIANYI2ZHOULINGDEZIJI,ZAIMIANYIHOU3ZHOUFAXIANXUEQINGIgGSHENGGAO,CISHIDIANYANDIBI、JIROUZHUSHEHUOJINGMAIZHUSHE100 CLD50XINCHENGYIBINGDUTexasZHUJINXINGGONGJI,ZAIGONGJIHOU10 dFAXIAN3GEGONGJIZUDECUNHUOLVJUNWEI100%(10/10),ERDUIZHAOZUWEI0(0/10)。

  2.6 重组传染性法氏囊病病毒

  CHUANRANXINGFASHINANGBINGBINGDU(Infectious bursal disease virus,IBDV)SHIYIZHONGRNABINGDU,JINZAISUZHUXIBAODEBAOJIANGFUZHI,JIYINZUJIAOXIAO,JINCHANSHENGSHAOLIANGDANBAI,DUIREWENDING,RONGYISHENGCHAN,KETONGGUOYINSHUIHUOPENSAMIANYI。JUBAODAOIBDVKEBIAODAKOUTIYIBINGDUDEVP2DANBAIHEBINGXINGGANYANBINGDUDEXIBAOBIAOWEIDENG[46,47].LiDENG[48]JIANGHNJIYINCHARUpIBDVDEpIBDV-HN,JIANGQIZHUANHUACHUANRANXINGFASHINANGBINGBINGDUGtZHU,RANHOUGANRANDF1XIBAO,SHAIXUANZHONGZUBINGDU,Western blotSHIYANBIAOMINGGANRANXUEQINGKEJIEHErIBDV-HNBIAODADE74 kDaDEHNDANBAI。JIANG105 TCID50YIMIAODIANYANDIBIMIANYI14 dLINGDELAIHANGJI,TISHIMIANYIZUDEXUEQINGIgGZAIJIEZHONGHOU2~3ZHOUSHANGSHENG,ZAIJIEZHONGHOU3ZHOUSHANGSHENGDAODAJIAOGAOCHENGDU,ZAIJIEZHONGHOU3ZHOUYONG103 ELD50CHUANRANXINGFASHINANGBINGBINGDUGXZHUDIANYANDIBIJINXINGGONGJI,ZAIGONGJIHOU10 dFAXIANMIANYIZUHEDUIZHAOZUDECUNHUOLVFENBIEWEI90%(9/10)HE0(0/10)。

  2.7 重组传染性气管炎病毒

  CHUANRANXINGQIGUANYANBINGDU(Infectious bronchitis virus,IBV)SHIYIZHONGRNABINGDU,JIYINZUDAXIAOWEI27.6 kb,HANYOUXUDUOFEIBIXUQU,KECHARUWAIYUANJIYIN,ERBUYINGXIANGBINGDUDEFUZHIHESHENGWUXUETEZHENG,KEZUOWEIYIMIAOZAITI[49].YangDENG[50]JIANGHNJIYINCHARUpMD19DEpMD-HN,YUpIBVZHONGZUDEpIBV-HN,JIANGQIZHUANHUACHUANRANXINGQIGUANYANBINGDUH120ZHUHOUZAIZHUANRANBHK-21XIBAO,SHAIXUANZHONGZUCHUANRANXINGQIGUANYANBINGDU,YICONGrIBV-HNCHOUTIDEZONGRNAWEIMOBAN,CAIYONGRT-PCRFANGFAKEKELONGCHU1 838 bpDEHNJIYINPIANDUAN。JIANG105 EID50YIMIAODIANYANDIBIMIANYI107 dLINGDEZIJI,ZAIMIANYIHOU2ZHOUFAXIANXUEQINGIgGSHENGGAO。CISHIYONG10 EID50DECHUANRANXINGQIGUANYANBINGDU41ZHUDIANYANDIBIJINXINGGONGJI,ZAIGONGJIHOU2ZHOUFAXIANMIANYIZUHEDUIZHAOZUDECUNHUOLVFENBIEWEI90%(9/10)HE50%(5/10)。

  3、结 语

  SUIRANGUONEIWAIXUEZHEBAODAODEWEISHENGWUJIEDAODEXINCHENGYIBINGDUYIMIAOMIANYIYIXIEDONGWUKECHANSHENGYIDINGDEBAOHULI,DANTAMENYOUDAODEBAOHUXINGMIANYIYINGDADESHUIPINGJIAODI,SHANGWEIDADAORENMENSUOYAOQIUDECHENGDU。RENMENFAXIANZHONGZURUSUANRUQIUJUNMIAOYIYUPEIYANG,ZHUANHUAXIAOLVGAO,ZHONGFUXINGHAO,DANZHANGQIDIJILIANGKOUFUGAIYIMIAOYOUKENENGYOUDAOMIANYINAISHOU;SHUSHANGHANSHAMENJUNMIAODEBIAODACHANWUYUTIANRANDANBAICUNZAICHAYI,BIAODASHUIPINGJIAODI;ZHONGZUDANZENGLISITEJUNZISHENHANYOUDEKANGSHENGSUKANGXINGJIYINKENENGDUIRENTIYOUHAI,BIAODADANBAIDESHUIPINGJIAODI,BIAODACHANWUYUTIANRANDANBAICUNZAIJIAODACHAYI;ZHUANJIYINZHIWUYIMIAODEZHILIANGKONGZHIHERENZHENGJIAONAN,PUBIANBUBEIGONGZHONGRENKEHEJIESHOU。TONGCHANGBINGDUZAITIDEZISHENDANBAIJIQISHUANGLIANRNAKEZUOWEIMIANYIZUOJI,DANBINGDUZAITICUNZAIQIANZAIDEZHIAIHEZHIBINGXING。SUZHUTINEIPUBIANCUNZAIKANGBINGDUDEKANGTI,ZAOXIANCUNZAIDEKANGBINGDUKANGTIKEYIZHIHOUXUJIEZHONGDEMIANYIYINGDA。BINGDUZAITIDEBAXIANGXINGCHA,CHANGYINQIGANRANDEBAQIGUANHUOFEIBAQIGUANCHANSHENGBINGBIAN,YINGXIANGMIANYIXIAOGUO。

赢发彩票代理   XIANDAISHENGWUXUEJISHUDEFAZHANRIXINYUEYI,SHIBITUIDONGXINCHENGYIBINGDUDEJIYINZUXUE、DANBAIZHIZUXUE、DAIXIEZUXUE、ZHUANLUZUXUEYIJIBIAOGUANYICHUANXUEDEYANJIUJINZHAN。ZHEIXIEXUEKEDEJINZHANDUIYUCHANMINGXINCHENGYIBINGDUHN/FDENGDANBAIDEKANGYUANJIEGOUYUGONGNENGDEGUANXI、SHAIXUANXINDEKANGYUANFENZIYIJIGOUJIANYOUGEQIKANGYUANFENZIZUCHENGDEDUOQIDUOJIAYIMIAOJUNJUYOUZHONGYAODEZHIDAOYIYI;XUYAOSHENRUYANJIUZHEIXIEYIMIAODEMIANYIYUANXING、ZHUANHUAXIAOLV、MHCXIANZHIXINGYIJINENGFOUZHANGQIZAISUZHUTINEIBIAODADENGZHUDUOWENTI;SHANGXUYANZHIXINXINGZUOJI、YIMIAOZAITIHEZHIBEIRONGHEKANGYUAN,CONGERTIGAOYIMIAODEMIANYIYUANXING;SHANGXUTANTAOZAISUZHUTINEIZHENGQUEPINGJIAXINXINGYIMIAOFENZIDEBAOHULIJIQIMIANYIJIZHIDEFANGFA;JIJIKAIZHANXINCHENGYIBINGDUKANGYUANDANBAIJIAZUJIQITAXIANGGUANDANBAISHENGWUXUEGONGNENGYANJIU,CONGERTIGAOYIMIAODESHEJISHUIPING。QIDAIZHEIXIEJIAODIANWENTIDECHANMINGTUIDONGZAITIJIEDAODEXINCHENGYIBINGDUYIMIAODEYANJIUJINRUYIGEXINGAODU。

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